| Pos | Club | P | W | D | L | GF | GA | GD | Pts | |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Brighton + Hove Albion | 46 | 28 | 8 | 10 | 73 | 35 | 38 | 92 | pr |
| 2 | Cardiff City | 46 | 23 | 13 | 10 | 95 | 58 | 37 | 82 | pr |
| 3 | Chesterfield (-9) | 46 | 25 | 14 | 7 | 79 | 42 | 37 | 80 | pr |
| 4 | Hartlepool United | 46 | 21 | 14 | 11 | 71 | 54 | 17 | 77 | pl |
| 5 | Leyton Orient | 46 | 20 | 15 | 11 | 59 | 51 | 8 | 75 | pl |
| 6 | Hull City | 46 | 19 | 17 | 10 | 47 | 39 | 8 | 74 | pl |
| 7 | Blackpool | 46 | 22 | 6 | 18 | 74 | 58 | 16 | 72 | ppl |
| 8 | Rochdale | 46 | 18 | 17 | 11 | 59 | 48 | 11 | 71 | |
| 9 | Cheltenham Town | 46 | 18 | 14 | 14 | 59 | 52 | 7 | 68 | |
| 10 | Scunthorpe United | 46 | 18 | 11 | 17 | 62 | 51 | 11 | 65 | |
| 11 | Southend United | 46 | 15 | 18 | 13 | 55 | 53 | 2 | 63 | |
| 12 | Plymouth Argyle | 46 | 15 | 13 | 18 | 54 | 61 | -7 | 58 | |
| 13 | Mansfield Town | 46 | 15 | 13 | 18 | 62 | 71 | -9 | 58 | |
| 14 | Macclesfield Town | 46 | 14 | 14 | 18 | 51 | 62 | -11 | 56 | |
| 15 | Shrewsbury Town | 46 | 15 | 10 | 21 | 49 | 65 | -16 | 55 | |
| 16 | Kidderminster Harriers | 46 | 13 | 14 | 19 | 47 | 61 | -14 | 53 | |
| 17 | York City | 46 | 13 | 13 | 20 | 42 | 63 | -21 | 52 | |
| 18 | Lincoln City | 46 | 12 | 15 | 19 | 57 | 64 | -7 | 51 | |
| 19 | Exeter City | 46 | 12 | 14 | 20 | 40 | 58 | -18 | 50 | |
| 20 | Darlington | 46 | 12 | 13 | 21 | 44 | 56 | -12 | 49 | |
| 21 | Torquay United | 46 | 12 | 13 | 21 | 52 | 77 | -25 | 49 | |
| 22 | Carlisle United | 46 | 11 | 15 | 20 | 41 | 65 | -24 | 48 | |
| 23 | Halifax Town | 46 | 12 | 11 | 23 | 54 | 68 | -14 | 47 | |
| 24 | Barnet | 46 | 12 | 9 | 25 | 67 | 81 | -14 | 45 | r |
| Date | Opponent | Score | Att. | ||||
|---|---|---|---|---|---|---|---|
| 14 July 2000 | Away | Gravesend + North. | Friendly | 1–1 | D | 0 | → |
| 18 July 2000 | Away | Kettering Town | Friendly | 2–0 | W | 0 | → |
| 22 July 2000 | Away | Billericay Town | Friendly | 0–0 | D | 0 | → |
| 25 July 2000 | Home | Gillingham | Friendly | 1–3 | L | 0 | → |
| 29 July 2000 | Home | Saffron Walden T | Friendly | 8–0 | W | 0 | → |
| 1 August 2000 | Home | Charlton Athletic | Friendly | 0–2 | L | 0 | → |
| 1 August 2000 | Home | Ex-Blues v. All Stars | Friendly | 2–2 | D | 0 | → |
| 7 August 2000 | Away | Barking | Friendly | 0–2 | L | 0 | → |
| 12 August 2000 | Home | Brighton + H.A. | League | 2–0 | W | 7492 | → |
| 19 August 2000 | Away | Torquay United | League | 1–1 | D | 2528 | → |
| 22 August 2000 | Home | Birmingham City | League Cup | 0–5 | L | 3694 | → |
| 26 August 2000 | Home | Darlington | League | 0–2 | L | 3444 | → |
| 28 August 2000 | Away | Cardiff City | League | 2–2 | D | 7628 | → |
| 2 September 2000 | Away | Lincoln City | League | 0–3 | L | 2691 | → |
| 5 September 2000 | Away | Birmingham City | League Cup | 0–0 | D | 9507 | → |
| 9 September 2000 | Home | Plymouth Argyle | League | 2–2 | D | 3417 | → |
| 12 September 2000 | Home | Kidderminster H. | League | 1–1 | D | 2403 | → |
| 16 September 2000 | Away | Halifax Town | League | 1–0 | W | 1447 | → |
| 23 September 2000 | Home | Scunthorpe United | League | 1–0 | W | 3110 | → |
| 30 September 2000 | Away | Rochdale | League | 1–0 | W | 3264 | → |
| 8 October 2000 | Away | Blackpool | League | 2–2 | D | 3915 | → |
| 14 October 2000 | Home | York City | League | 1–0 | W | 4724 | → |
| 17 October 2000 | Home | Barnet | League | 2–0 | W | 4124 | → |
| 21 October 2000 | Away | Hull City | League | 1–1 | D | 6701 | → |
| 24 October 2000 | Home | Exeter City | League | 1–1 | D | 4942 | → |
| 28 October 2000 | Away | Mansfield Town | League | 1–1 | D | 2200 | → |
| 4 November 2000 | Home | Macclesfield Town | League | 3–1 | W | 4910 | → |
| 11 November 2000 | Away | Carlisle United | League | 1–3 | L | 2201 | → |
| 18 November 2000 | Away | Torquay United | FA Cup | 1–1 | D | 2171 | → |
| 25 November 2000 | Home | Cheltenham | League | 0–1 | L | 4199 | → |
| 28 November 2000 | Home | Torquay United | FA Cup | 2–1 | W | 3877 | → |
| 2 December 2000 | Away | Hartlepool United | League | 0–1 | L | 2638 | → |
| 5 December 2000 | Home | Cheltenham | Trophy | 2–0 | W | 1000 | → |
| 10 December 2000 | Away | Canvey Island | FA Cup | 2–1 | W | 11402 | → |
| 16 December 2000 | Home | Chesterfield | League | 3–2 | W | 3889 | → |
| 22 December 2000 | Away | Shrewsbury Town | League | 1–0 | W | 2499 | → |
| 26 December 2000 | Home | Leyton Orient | League | 0–1 | L | 9950 | → |
| 30 December 2000 | Home | Torquay United | League | 1–1 | D | 3643 | → |
| 1 January 2001 | Away | Brighton + H.A. | League | 2–0 | W | 6503 | → |
| 6 January 2001 | Home | Kingstonian | FA Cup | 0–1 | L | 7270 | → |
| 9 January 2001 | Home | Cambridge United | Trophy | 3–1 | W | 1694 | → |
| 13 January 2001 | Home | Cardiff City | League | 1–1 | D | 4601 | → |
| 20 January 2001 | Away | Leyton Orient | League | 2–0 | W | 6859 | → |
| 27 January 2001 | Home | Shrewsbury Town | League | 0–0 | D | 4289 | → |
| 30 January 2001 | Home | Bristol Rovers | Trophy | 1–0 | W | 2192 | → |
| 3 February 2001 | Home | Lincoln City | League | 1–0 | W | 3889 | → |
| 13 February 2001 | Home | Swindon Town | Trophy | 2–1 | W | 3337 | → |
| 17 February 2001 | Home | Halifax Town | League | 0–3 | L | 3746 | → |
| 20 February 2001 | Away | Kidderminster H. | League | 1–2 | L | 2781 | → |
| 24 February 2001 | Away | Scunthorpe United | League | 1–1 | D | 2631 | → |
| 3 March 2001 | Home | Rochdale | League | 3–0 | W | 3681 | → |
| 6 March 2001 | Away | York City | League | 0–1 | L | 2144 | → |
| 10 March 2001 | Home | Blackpool | League | 0–3 | L | 4810 | → |
| 13 March 2001 | Home | Brentford | Trophy | 1–2 | L | 5055 | → |
| 17 March 2001 | Away | Barnet | League | 1–2 | L | 2654 | → |
| 20 March 2001 | Away | Brentford | Trophy | 1–2 | L | 6579 | → |
| 31 March 2001 | Away | Chesterfield | League | 1–1 | D | 4018 | → |
| 3 April 2001 | Away | Darlington | League | 1–1 | D | 2782 | → |
| 7 April 2001 | Home | Hartlepool United | League | 2–1 | W | 3759 | → |
| 14 April 2001 | Away | Exeter City | League | 2–2 | D | 4535 | → |
| 21 April 2001 | Away | Macclesfield Town | League | 0–1 | L | 1594 | → |
| 24 April 2001 | Away | Plymouth Argyle | League | 3–3 | D | 3619 | → |
| 28 April 2001 | Home | Carlisle United | League | 1–1 | D | 4175 | → |
| 1 May 2001 | Home | Hull City | League | 1–1 | D | 3573 | → |
| 5 May 2001 | Away | Cheltenham | League | 1–2 | L | 3637 | → |
| 8 May 2001 | Home | Mansfield Town | League | 3–1 | W | 3345 | → |